abstract |
An apparatus and method for analyzing at least two analytical items in a sample solution in a single container, wherein a series of transparent containers supported on a rotatable disc pass over a sample-receiving position, a first reagent solution-adding position, a second reagent solution-adding position, an optical path of a photometer, a solution-discharging device and a washing device. In the sample-receiving position, a fixed volume of sample is introduced into one of the transparent containers. In the first reagent solution-adding position, the first reagent solution is introduced into the transparent container, whereby a reaction is allowed to proceed. Subsequently, the container is passed across an optical path, and absorbance of light by the reaction solution is measured, to provide first measurements. Thereafter, a second reagent solution is introduced into the transparent container at the second reagent solution-adding position, without removal of the first reaction solution, whereby a further reaction is allowed to proceed. Subsequently, the container is passed across an optical path, and absorbance of light by the resulting solution in the transparent container is measured, to provide second measurements. The first analytical item can be investigated from the first measurements, and the second analytical item can be investigated from differences between the first and second measurements. |