| abstract |
Disclosed herein is a novel compound which is excellent in solubility to water around a neutral range, stable in an aqueous solution state for a long period of time, and high in coloring sensitivity with a stable coloring development, and is represented by the general formula (I): <IMAGE> (I) wherein R1, R2, R3 and R4, which may be the same as or different from one another, represent lower alkyl groups, and X1 and X2 both represent -O(CH2)nSO3M in which M is a hydrogen atom, an alkali metal ion or NH4+, and n is an integer of 2-4, or either of X1 and X2 represents -O(CH2)nSO3M in whch M and n are the same meanings as given above and the other represents a hydrogen atom. This reagent is hardly susceptible of the influence of the serum components such as hemoglobin, bilirubin and so on due to its color having absorption on a longer wavelength side. Disclosed is also a method of quantitatively measuring an oxidative substance, particularly, hydrogen peroxide, by using this color test reagent. |