| abstract |
PCT No. PCT/US81/00576 Sec. 371 Date May 17, 1982 Sec. 102(e) Date May 17, 1982 PCT Filed Apr. 29, 1981.A process of isolating and identifying cloned DNA segments from defined regions of a chromosome by preparing a series of cell hybrids from a first species and a second species, which hybrids contain a chromosome or deletion mutant of a chromosome as the only chromosomal component of the second species, constructing a library of recombinant clones of DNA from the hybrid cell, plaquing the library on culture plates, transferring the plaques onto a medium which is capable of holding DNA in a covalent or noncovalent fashion, hybridizing the medium to total (nick-translated) radioactive second species DNA, and collecting the radioactive 2d species plaques. |