| abstract |
A medium is provided which is useful in a fast and convenient system for multiplying cassava plants vegetatively by means of in vitro micropropagation of nodal cultures. This medium includes in parts per million the following: -about 1200 NH4NO3 -from about 1000-1500 KNO3 -about 300 MgSO4.7H2O -from about 150-300 NaH2PO4.H2O -from about 150-300 CaCl2.2H2O -from about 18.5-37 Na2.EDTA -from about 13.9-27.8 FeSO4.7H2O. - and suitable amounts of essential ingredients for a cassava micropropagation medium. In paticular, the medium also contains in part per million about: 3.0 H3BO3, 10.0 MnSO4.H2O, 6.0 ZnSO4.7H2O, 0.25 Na2MoO4.2H2O, 0.025 CuSO4.5H2O, 0.025 CaCl2.6H2O, 0.75 KI, 2.5 Nicotinic acid, 10.0 Thiamine HCl, 1.0 Pyridoxine HCl, 100.0 M-inositol, 0.5 Glycine, 0.5 Folic acid, 0.05 Biotin, 0.5 D-Ca-Pantothenate, 0.25 Riboflavin, 0.5 Ascorbic acid, 0.1 choline chloride, 1.0 L-cysteine HCl, 10.0 Malic acid and 50.0 Casein Hydrolysate. Furthermore, the medium desirably contains about 3 percent sucrose or glucose. |