| abstract |
A method of in vitro clonal propagation of plantlets from excised gymnosperm tissue in which the excised tissue is pulse treated on a nutrient medium containing at least about 10 mg/L, preferably at least about 20 mg/L, of a cytokinin for a time sufficient to induce formation of adventitious buds on the excised tissue and is then transferred to a nutrient medium free of exogenous growth factors and maintained thereon until the induced adventitious buds produce rootable shoots. The rootable shoots may then be rooted by conventional means but preferably are pulse treated on a nutrient medium containing a phenolic compound, preferably in an amount of at least about 5 mg/L, and an auxin, preferably in an amount of at least about 5 mg/L, for a time sufficient to induce formation of adventitious roots and then transferred to a nutrient medium free of exogenous growth factors until the shoots are rooted. |