http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-4374930-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5d9f3ca41550d315642580237250c5b0 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-815 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0006 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-04 |
| filingDate | 1981-10-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1983-02-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_50d6605a3a9f56817efa8096023d8f02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b20be7b140b8cb7452585663aa3760f1 |
| publicationDate | 1983-02-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-4374930-A |
| titleOfInvention | Method for the purification of cholesterol oxidase |
| abstract | A method for the purification and recovery of cholesterol oxidase is disclosed. Cholesterol oxidase is purified by a method including the steps of: (a) contacting a solution of the cholesterol oxidase to be purified with a chromatography composition comprising an unbound water-insoluble steroid which is a nonsubstrate for cholesterol oxidase so as to adsorb the cholesterol oxidase on the steroid; (b) separating the solution from the steroid; and (c) contacting the steroid with an eluting solution containing a surfactant at a concentration between 0.001 percent and 10 percent so as to recover the adsorbed cholesterol oxidase. In a preferred embodiment, the solution containing the cholesterol oxidase to be purified is a microorganism growth medium having cholesterol oxidase activity and, prior to the step of contacting the solution with a steroid, the solution is contacted with an ion-exchange chromatography material. In yet another embodiment, the eluting solution containing the surfactant also contains a metal salt of a concentration of at least about 0.005 molar. There is also disclosed a method for forming a packed bed of a composition comprising the unbound steroid which comprises the step of first suspending the steroid in a water-miscible organic solvent. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5916759-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5905143-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5171681-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5238816-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5206148-A |
| priorityDate | 1981-10-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 114.