| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_24295419fdc938ca80a28f7cf0db1781 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S435-803 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N59-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N59-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-24 |
| filingDate |
1980-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
1982-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9877e1c15bd22fcc793db57dcb396d85 |
| publicationDate |
1982-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-4366245-A |
| titleOfInvention |
Method for the isolation of symbiotic microorganisms from host plants using an osmium tetraoxide fixative |
| abstract |
Symbiotic microorganisms such as nitrogen fixing bacteria and phosphorus-providing fungi are isolated from nodules and mycorrhiza on the roots of host plants by sterilizing the surface and subsurface areas of root tissue with an aqueous solution of a fixative containing osmium tetraoxide. The osmium tetraoxide is highly soluble in water, and volatilizes at room temperature to form a vapor which penetrates subsurface air pockets in root tissues to kill contaminating microorganisms. The osmium tetraoxide also provides a visual indicator of its progress through plant tissue by a progressive darkening of such tissue from the surface of root samples toward their centers. In this manner, the sterilization can be halted before the centrally located causitive bacteria is destroyed. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-4861721-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6204030-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6569639-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5843657-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5843644-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6867038-B2 |
| priorityDate |
1980-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |