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filingDate 1981-05-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 1982-10-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-4355110-A
titleOfInvention Preparation of debranching enzyme from rice for producing a low calorie beer
abstract A debranching enzyme (pullulanese) useful in the preparation of a low calorie beer may be obtained from rice by extraction of the rice with an aqueous buffer system having a pH of about 6. A preferred buffer system is 0.1 M potassium phosphate-0.2 M-sodium chloride. Extraction is preferably carried out at a temperature of about 50 DEG C. for about 3 hours. When malted rice is used as the enzyme source a particularly useful mixture of the debranching enzyme and alpha 1,4 carbohydrases is obtained.
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