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filingDate 1981-01-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 1982-10-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d0a891109ebb318613cb91717e791201
publicationDate 1982-10-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-4355106-A
titleOfInvention Continuous process for the production of gelable exopolysaccharide
abstract The present invention is directed to a two-stage continuous process for the production of a gelable curdlan-type exopolysaccharide. In the first stage a stable, curdlan-producing strain of microorganism such as Alcaligenes faecalis var. myxogenes ATCC 31749 and ATCC 31750, is grown aerobically in an aerated, agitated culture medium containing assimilable carbon, nutrients and organic salts. The amount of nitrogen in the first stage is so limited that the effluent therefrom contains substantially no inorganic nitrogen. The effluent is introduced into a second stage in a constant volume fermenter wherein it is mixed with a nitrogen-free carbohydrate. The resultant mixture is aerated and mixed at pH 5.5 to 6.5 at a temperature of from 25° to 35° C., the volume and dilution rate in the reactor being selected so that the residence time of the microorganism in the fermenter does not exceed an equivalent to the maximum length of time during which the activity of a batch culture of the microorganism with respect to product synthesis is maximal. The product is subsequently isolated.
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