| abstract |
A dry stain for reticulocyte staining comprising a mixture of new methylene blue N dye, potassium acetate, and an anti-blood-clotting agent. The stain is prepared in a concentrated aqueous solution, filtered, dispensed volumetrically into tubes, and dried. The correct quantity of dye per tube is determined spectrophotometrically, and the proper ratio of dye to potassium acetate is determined by pH measurements. For staining reticulocytes, whole blood is added to the tube and mixed with the dry stain. Smears are then prepared on slides in a conventional manner for microscopic examination. |