| abstract |
In vitro net biosynthesis of chlorophyll and grana involving: irradiation of etiolated plant tissue; homogenization, in an environment including available oxygen, of the tissue in a buffer comprising biosynthetic cofactors including coenzyme A, glutathione, potassium, inorganic phosphate, methyl alcohol, magnesium, nicotinamide adenine dinucleotide and adenosine triphosphate; isolation from the homogenate of developing chloroplasts; irradiated incubation of the isolated chloroplasts in the presence of oxygen in another buffer of a constitution similar to that above-described and including delta -aminolevulinic acid. Synthesized products are isolated and quantified. |