| abstract |
An enzyme reagent formulation for use in assaying a biological specimen for uric acid is prepared as a granular, water-soluble, anhydrous, storage-stable mixture containing uricase, potassium chloride, mannitol, gum acacia, bovine serum albumin, glycine, sodium carbonate and a nitrogen containing polyoxyalkylene nonionic surfactant obtained by the sequential reaction of ethylenediamine with propylene oxide and ethylene oxide in the presence of a catalyst. The surfactant contains polyoxypropylene chains having an average molecular weight of between about 750 and about 6750, and polyoxyethylene chains constituting between about 10 and about 80 weight percent of the surfactant. The surfactant has an advantageous effect upon granulation, dissolution and storage stability of the reagent formulation. |