http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-3899397-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3d3be3c4e131833724478138c489539c |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-52 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-52 |
| filingDate | 1973-07-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1975-08-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7b8c133a69020fe6580ff8a2508baf48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e98194de785c6205761358a53d332fb3 |
| publicationDate | 1975-08-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-3899397-A |
| titleOfInvention | Glutamic oxalacetic transminase assay method |
| abstract | An improved diazonium coupling procedure for L-aspartate: 2oxoglutarate amino transferase, EC 2.6.1.1, or glutamicoxalacetic transaminase (GOT) assay. The method for determining the glutamic-oxalacetic transaminase in biological fluids involves preparing a substrate of 1-aspartic acid, Alpha ketoglutaric acid, buffer, surfactant and water. The substrate has a pH between about 5 and about 8, preferably 7.5. A sample of biological fluid is admixed with substrate. The admixture is incubated. Then an aprotic solvent is added to the admixture to stop the reaction between the 1-aspartic acid and Alpha ketoglutaric acid which is catalyzed by the glutamic-oxalacetic transaminase. A diazonium dye and methanol is then added to the admixture. The activity level of the glutamic-oxalacetic transaminase by measuring the absorbance against a reagentspecimen blank is determined. The preferred diazonium dye is Diazo Red RC. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-4271265-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003100029-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2004146852-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-4801535-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-4309184-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-8705633-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5041373-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7094558-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-3985621-A |
| priorityDate | 1973-07-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 148.