| abstract |
A new and superior method of producing cells in suspension culture is carried out by culturing the cells in a new culture medium under suitable agitation in a culture vessel which presents resinous, organic, polymeric surfaces to the medium. Blood serum is an ingredient of the medium. The temperature and pH of the medium are those regularly used in cell culture. The cells remain in the culture without passaging, cells being removed as necessary to keep the cell population at about 105 to about 106 cells/ml. Medium is periodically removed and replaced with fresh medium, and aggregated cells are periodically dispersed. |