http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-3769173-A
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_05ff905fdc49089f5f25746eaeb40190 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2337-12 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-37 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-37 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48 |
filingDate | 1972-08-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 1973-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_48f725e4642275b0cc015f19766b9e23 |
publicationDate | 1973-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-3769173-A |
titleOfInvention | Determination of gamma-glutamyl transpeptidase in biological fluids |
abstract | A method for determining gamma-glutamyl transpeptidase in biological fluids is described which comprises incubating the test fluid with an aqueous buffered solution of an L-gammaglutamyl-p-nitroanilide substrate, glycylglycine, sodium nitrite, and optionally, magnesium chloride hexahydrate, for a predetermined period of time; adding, after incubation is completed, a mineral acid reagent solution of 8-hydroxy quinoline sulfate containing a water soluble, nonionic emulsifier, the mineral acid solution having a pH of between 0.8 and 1.3; adding an alkaline solution which will impart a pH of between 10.5 and 12, in the total reaction solution, said alkaline solution optionally containing ethylenediaminetetraacetic acid, tetrasodium salt; and reading the optical density of the total reaction solution as a measure of gamma-glutamyltranspeptidase concentration in the test sample of biological fluid. The method of the invention is accurate and approximately 4.5 times more sensitive than the standard test method currently being used. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-4336331-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-4448715-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-4116774-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2005266579-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6235493-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2003166038-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-4511651-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5506114-A |
priorityDate | 1972-08-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 518.