abstract |
INSOLUBLE ENZYMES ARE PREPARED BY ESTERIFYING A CARBOXYL MOIETY WITH A COMPOUND CAPABLE OF FORMING AN ACTIVATED ESTER WITH THE CARBOXYL MOIETY AND THEN CONDENSING THE ACTIVATED ESTER WITH AN AMINE MOIETY. AT LEAST A PORTION OF EITHER THE CARBOXYL MOIETY OR THE AMINE MOIETY MUST BE AN ENZYME. EXEMPLARY OF THE ESTER FORMING COMPOUND IS N-ETHYL-5-PHENYL ISOOXAZOLIUM-3-SULFONATE. |