http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-3670075-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a0f72c66096c79f62800a395826ee550 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-57 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-57 |
| filingDate | 1970-05-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 1972-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_08c04f003111d163243c879dd4d8830c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9c1a8e31223a83696e633e057c273ec3 |
| publicationDate | 1972-06-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-3670075-A |
| titleOfInvention | Process of preparing a protease inhibitor |
| abstract | Process for preparing a protease inhibitor from animal pancreas, for instance hog and cattle pancreas, following extraction of insulin therefrom with a dilute mineral acid, such as phosphoric acid, comprising the steps of: 1. EXTRACTING MOIST CUT-UP PANCREAS MATERIAL WITH 60 TO 70 PERCENT AQUEOUS ETHANOL HAVING A PH of 10-13, 2. REMOVING THE ETHANOL FROM THE EXTRACT BY EVAPORATION UNDER DECREASED PRESSURE AND AT A TEMPERATURE NOT EXCEEDING 40* C., 3. cooling the remaining concentrated extract whereby lipoids present therein are separated out and removing the separated lipoids, 4. INTRODUCING A WATER-SOLUBLE INORGANIC SALT AND PREFERABLY AMMONIUM OR MAGNESIUM SULFATE, WHEREBY A PRECIPITATE COMPRISING AN PROTEIN COMPLEX IS SEPARATED OUT AND RECOVERING THE PRECIPITATE, 5. FORMING A SUSPENSION OF THE PRECIPITATE FROM STEP 4 IN WATER HAVING A PH of 5.5 to 7.5 and heating the suspension to a temperature of 50* to 80* C, introducing trichloracetic or sulfosalicylic acid into the suspension in a concentration of 2 to 3 weight percent, cooling the resulting mixture to a temperature of 15* to 20* C., whereby the protein is separated out as a precipitate and separating off said albumin precipitate, 6. ADJUSTING THE PH of the filtrate to a pH of 7.0 to 7.5, saturating the filtrate with sodium chloride in an amount of 25 to 30 g/100 ml filtrate and acidifying the saturated filtrate with hydrochloric acid to provide a pH of 1.0 to 3.0 whereby the inhibitor is separated out as a precipitate, 7. SUBJECTING THE PRECIPITATE FROM STEP 6 TO SUCCESSIVE WASHINGS WITH A BUFFERED SODIUM CHLORIDE SOLUTION WHOSE PH is successively increased from 1.7 to 9.0 and separately recovering the fractions from each washing, combining the fractions recovered in the pH range of 3.0 to 6.0, subjecting the combined fractions to deionization and recovering the pure inhibitor. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2007036777-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2004132068-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2010273170-A1 |
| priorityDate | 1969-05-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 155.