| abstract |
PROCESS FOR THE DETERMINATION OF HYDROXYPROLINE IN BIOLOGICAL FLUIDS, SUCH AS SERUM, URINE, LUMBAR FLUID AND INTERARTICULAR FLUID. IT COMPRISES TREATING A SAMPLE WITH A STRONG ACIDIC CATION EXCHANGE RESIN, WHICH ADSOBS THE FREE AND PEPTIDE-BOUND HYDROXYPROLINE AND HYDROLYZING THE ABSORBED PIPTIDE-BOUND HYDROXYPROLINE BY HEATING, WHEREAFTER THE HYDROXYPROLINE IS ELUTED AND DETERMINED BY MEANS OF A COLOUR REACTION. BESIDES, THE INVENTION COMPRISES A DIAGNOSTIC PACK CHIEFLY COMPRISING A BOTTLE WITH SAID RESIN, A TUBE FOR THE PERFORMANCE OF THE ADSORPTION AND HYDROLYSIS, A BOTTLE WITH A STANDARD QUANTITY OF HYDROXYPROLINE AND A REAGENT FOR THE PERFORMANCE OF A COLOUR REACTION. IN THIS WAY TRACING OF DEVIATIONS IN COLLAGEN METABOLISM CAN EASILY BE PREFORMED. |