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filingDate 1968-12-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 1971-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_659975a295dbdce023e2e95e1020860c
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publicationDate 1971-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-3579423-A
titleOfInvention Preparation of tissue culture media
abstract A POWDERED NUTRIENT COMPOSITION IS PROVIDED CONTAINING MOST OF THE NUTRITION REQUIREMENTS FOR CULTURING ANIMAL CELLS AND AN ACIDIC BUFFERING AGENT TO MAINTAIN THE PH OF AN AQUEOUS SOLUTION OF THE COMPOSITION AT 3.5-5.0. THIS COMPOSITION CAN BE DISSOLVED IN WATER AND STERILIZED BY AUTOCLAVING PRIOR TO ITS INCORPORATION IN A TISSUE CULTURE MEDIUM. AFTER AUTOCLAVING OF THE NUTRIENT COMPOSITION, STERILE SOLUTIONS OF GLUTAMINE AND BICARBONATE IONS ARE ASEPTICALLY ADDED TO THE COMPOSITION TO FORM A TISSUE CULTURE MEDIUM.
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