| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d07a4ddba85ae3e35ca0a6f85dad9f6d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_77e58a46a7ba5008f1f8e888cf52eca8 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2537-164
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6804
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2535-122
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2522-101
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2545-101
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2545-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-166
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6874
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-154 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6804
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1082 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858 |
| filingDate |
2022-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_edcb6db247246cc93eb840c5c1a7fe9d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2b9b4e3255c38cc8a740f73a0333f2cb
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5ff4b99c170687b9a0ead58dc2832876
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_af0348ffaecdd9febe96b8f6e36989c7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2137d0f1fe3284596e041c4c39fbb962 |
| publicationDate |
2023-01-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2023024827-A1 |
| titleOfInvention |
Synthetic spike-in controls for cell-free medip sequencing and methods of using same |
| abstract |
There is described herein, a method of capturing and analyzing cell-free methylated DNA in a sample. The method involves subjecting the sample to library preparation to permit subsequent sequencing of the cell-free methylated DNA. A predetermined amount of control synthetic DNA fragments are added to the sample. The control synthetic DNA fragments each have a known nucleic acid sequence that does not align to a target genome sequence, and at least some of the control synthetic DNA fragments are methylated. The sample is denatured, and cell-free methylated DNA and the control synthetic DNA fragments are captured using a binder selective for methylated polynucleotides. The captured DNA is amplified and sequenced. |
| priorityDate |
2019-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |