| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4293d5e759e7a8c4422b3233f8a902ef |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-158 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6458
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6874
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6834 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6874 |
| filingDate |
2021-07-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5b78630cb57f48d1a0eac5fb62713f12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a1c9c1a557de28efc07dfa35f7ede241
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9f8c7950c2679754edf2ae48afbae7f5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6ef18ddfc5c4823a8985456e1fd8ca0a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9273d7f4dc569f24dca5648e05e45fd3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2c2c5f925904c16db663ecc6cce92a83
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b19b3e705ab406ebc26e7329ad991d75
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7302eaa8da19af3e1ccb1a9e3e5d2ece
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f06f4c78aa2685c416b7b0808471d406
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8f5eb071d43eb7b1c6b69eabad32865b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4491db66e2d3d3e6723dfa7b9a321684
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_db265eb9be11722dca320bcfd908dea7 |
| publicationDate |
2022-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2022403463-A1 |
| titleOfInvention |
Compositions and methods for pairwise sequencing |
| abstract |
The present disclosure provides compositions and methods that employ the compositions for conducting pairwise sequencing and for generating concatemer template molecules for pairwise sequencing. The concatemers can be generated using a rolling circle amplification reaction which is conducted either on-support, or conducted in-solution and then distributed onto a support. The rolling circle amplification reaction generates concatemers containing tandem copies of a sequence of interest and at least one universal adaptor sequence. An increase in the number of tandem copies in a given concatemer increases the number of sites along the concatemer for hybridizing to multiple sequencing primers which serve as multiple initiation sites for polymerase-catalyzed sequencing reactions. When the sequencing reaction employs detectably labeled nucleotides and/or detectably labeled multivalent molecules (e.g., having nucleotide units), the signals emitted by the nucleotides or nucleotide units that participate in the parallel sequencing reactions along the concatemer yields an increased signal intensity for each concatemer. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11773439-B2 |
| priorityDate |
2021-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |