http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2022381781-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_62f941494409aea84f621977c5f0ab81 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6804 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56983 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54388 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6804 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 |
| filingDate | 2020-10-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7929919bd2fba3362d0a28394b893f9f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_01042301049b4ec09415d29ee38c3e64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_94b6c8694b6622bc02aca1d1f37f60ee http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_03a71b5e6c3c3e90878ee4fbaea597c2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_319f97da01ab479d8a07fd4c42385b18 |
| publicationDate | 2022-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2022381781-A1 |
| titleOfInvention | Methods for ultrasensitive detection of protein and cellular biomarkers |
| abstract | CRISPR-based diagnostic methods and compositions are provided. One embodiment provides the use of DNA-barcoded antibodies or peptide-MHC (pMHC) tetramers (e.g., Kb-OVA257-264, Db-GP10025-33, Db-GP33-41) and CRISPR-Cas protein, and a guided DNA endonuclease, to achieve ultrasensitive detection of soluble and cell surface proteins. The disclosed embodiments can use type V: Cas12a; type VI: Cas13a, or Cas13b. Combining DNA encoding with CRISPR-Cas protein recognition is a sensitive system because barcodes can be isothermally amplified and Cas, for example Cas12a, enzymatically cleaves DNA reporters upon barcode detection, providing two rounds of amplification and enabling measurement of protein concentration by sample fluorescence or using by paper-based assays. This platform enables monitoring of protein and cellular biomarkers and further expands the toolbox of CRISPR/Cas-based technologies |
| priorityDate | 2019-10-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 745.