http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2022365084-A1

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filingDate 2020-10-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cae295603a4b91d77c1c0ac2f6ebc9bf
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publicationDate 2022-11-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2022365084-A1
titleOfInvention Immunochromatographic device for extracting and measuring sugar chain antigen
abstract This invention provides a method and an immunochromatographic device that enable measurement with sufficient sensitivity by performing nitrous acid extraction over a sufficiently long period of time in an immunochromatographic method comprising extracting a sugar chain antigen with nitrous acid and measuring the extracted sugar chain antigen on an immunochromatographic test strip. Such immunochromatographic device comprises an immunochromatographic test strip for extracting and measuring a sugar chain antigen in a specimen and a cassette for accommodating the test strip, wherein the immunochromatographic test strip comprises a sample pad to which a specimen mixed with the nitrite or acid solution is added, a label region comprising a labeled antibody obtained by labeling an antibody against the sugar chain antigen, and a detection region on which the antibody against the sugar chain antigen is immobilized, an antibody-sugar chain antigen-labeled antibody complex is formed in the detection region to measure the sugar chain antigen, the immunochromatographic test strip has a region impregnated with a neutralizing reagent upstream of the label region and further has, upstream of the region impregnated with the neutralizing reagent, a region impregnated with a solid acid reagent when the specimen mixed with the nitrite is used or a region impregnated with the nitrite when the specimen mixed with the acid solution is used, and the immunochromatographic device comprises a specimen dropping port on top of a sample pad of the test strip. The immunochromatographic device has: (i) a wide specimen dropping port to promote sugar chain antigen extraction with a nitrite and a solid acid reagent by retaining the added specimen sample solution and supplying the specimen sample solution to the region impregnated with the solid acid reagent or the nitrite within a short period of time; and (ii) no gaps between the dropping port and the sample pad so as to prevent the sample from leaking from the dropping port.
priorityDate 2019-10-04-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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