Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_41686a979f961bce6a9855b4b409a5b8 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6816 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6834 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1065 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6816 |
filingDate |
2022-05-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3453cc0874b8b9233ca32e74314fac55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f5c813549bd78a73a991809ee15d82f3 |
publicationDate |
2022-11-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-2022348904-A1 |
titleOfInvention |
Molecular barcoding on opposite transcript ends |
abstract |
Disclosed herein include systems, methods, compositions, and kits for molecular barcoding on the 5′-end of a nucleic acid target. After barcoding a nucleic acid target using an oligonucleotide barcode comprising a target binding region and a molecular label to generate a barcoded nucleic acid molecule, an oligonucleotide comprising a complement of the target binding region can be added to generate a barcoded nucleic acid molecule comprising the target-binding region and the complement of the target-binding region. A stem loop is formed with intra-molecular hybridization of the barcoded nucleic acid molecule, which can be extended to generate an extended barcoded nucleic acid molecule comprising the molecular label and a complement of the molecular label. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11702706-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11773436-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11661625-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11661631-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11782059-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11634708-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11639517-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11649497-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11739443-B2 |
priorityDate |
2018-05-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |