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filingDate 2022-07-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2022-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2022339554-A1
titleOfInvention Continuous Sample Purification Systems and Methods
abstract Sample purification systems include a particle extraction assembly having a mixing compartment and a settling compartment. A biological sample is mixed with two liquid phases formulated to effectuate transfer of a biological molecule into a first phase and particulate contaminants into a second phase. The first phase includes a solubilizing salt, the second phase includes an organic molecule, and the mixture can have little or no monoatomic salt or dextran. The molecule-containing first phase can be optionally concentrated without also concentrating the particulate contaminants and introduced into a multi-stage liquid-liquid extractor, by which the biological molecule or molecular contaminants are extracted from the first phase into a third phase, thereby purifying the molecule away from contaminants. The extracted sample can be further purified through a series of processing steps. The system can be run in continuously mode to maintain sterility of the sample.
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