http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2022333184-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_473c5c9b53e303173b1374acf4160ca9 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2535-122 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-155 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2531-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2523-109 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-131 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-191 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-07049 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1096 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6855 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1276 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6855 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 |
| filingDate | 2020-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dba7ef2214f6c393123869407fe1eaee http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5c81935e5fc4f06600bffaa5eda5cf1e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_267f4ee19942615f4efed39d8b8b03a5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_59527182161ca0b6b4ada7a25505974c |
| publicationDate | 2022-10-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2022333184-A1 |
| titleOfInvention | Method of amplifying mrnas and for preparing full length mrna libraries |
| abstract | An inventive method for amplifying at least one RNA which is contained in a sample includes reverse transcribing the at least one RNA using a first primer, adding a dideoxy nucleotide which is modified in the 3′-position with a first partner of a pair of azide and alkyne molecules by action of a template independent polymerase to attach a single 3′-azide- or 3′-alkyne-modified dideoxy nucleotide at the 3′-end of the obtained cDNA, adding an adapter molecule which comprises a polynucleotide sequence and at its 5′-end a second partner of such pair of azide and alkyne molecules and ligating the adapter to the 3′-modified cDNA under formation of a triazole linkage, adding a second primer which is complementary to at least a part of the adapter molecule and which contains at its 3′-end a nucleotide which is complementary to the dideoxy nucleotide at the 3′-end of the cDNA to effect hybridization and binding of the second primer overlapping the triazole linkage, adding a third primer and amplifying the full length cDNA. Variations of this method are also disclosed. Uses of such method especially for preparing a full length RNA library and for sequencing of a plurality of RNAs contained in a sample, as well as reagent kits for performing such methods are also disclosed and included in the invention. |
| priorityDate | 2019-12-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 301.