http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2022333170-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_df5c662baf778f221a5be650e3af5380 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-537 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-107 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6834 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1075 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6834 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 |
| filingDate | 2022-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eae42b4c2c1986fbc3ef3e932c7deb7b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9f9b8d48459539c17d61037ee200f7d6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c66390734db48e0b0a2f3124118f4245 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_830388c49a94d745e1c3004b121d538e |
| publicationDate | 2022-10-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2022333170-A1 |
| titleOfInvention | Method and apparatus for simultaneous targeted sequencing of dna, rna and protein |
| abstract | Provided herein are methods and systems for the simultaneous targeted detection and sequencing of DNA, RNA, and Protein. In typical embodiments, the DNA, RNA, and Proteins are detected, characterized, and sequenced using just a single mammalian cell. One embodiment of detecting and characterizing DNA, RNA, or protein from a mammalian cell includes encapsulating a single cell in a drop and performing a protease digest on the encapsulated cell drop, performing a reverse transcriptase reaction; performing a droplet merger with barcoding PCR reagents and barcoding beads; performing a PCR reaction to attach the cell barcodes to the DNA targeted amplicons, RNA targeted amplicons, and protein tag amplicons, where all amplicons from the same emulsion contain the same cell barcode; and detecting and characterizing a DNA, RNA, or protein amplicon by sequencing the cell barcode incorporated into each amplicon. |
| priorityDate | 2019-05-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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