http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2022325309-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_24aca9ded2638ea793d05360dde7a4a0 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y605-01003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
filingDate | 2019-08-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6ad5305caafaf98e6d24e902d410b8c6 |
publicationDate | 2022-10-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-2022325309-A1 |
titleOfInvention | Method for producing nucleic acid molecule |
abstract | The present invention provides a method for producing a single-stranded RNA, the method comprising:(I) a step of reacting a first single-stranded RNA having a phosphate group at the 5′-terminal and a second single-stranded RNA having a hydroxy group at the 3′-terminal with an RNA ligase classified into EC 6.5.1.3 determined by International Union of Biochemistry as an Enzyme Commission number and having a nick repair activity in a double-strand to link said first single-stranded RNA to said second single-stranded RNA; and(II) a step of purifying the reaction product comprising the single-stranded RNA produced in the step (I) by reverse-phase column chromatography using a mobile phase comprising at least one ammonium salt(s) selected from the group consisting of monoalkylammonium salts and dialkylammonium salts. |
priorityDate | 2019-08-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 767.