http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2022316000-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e52b8296a4d53ea0a5aa789815c7d95f http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c190c99174dfe46deef72ae3ad792590 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6876 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6851 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6876 |
filingDate | 2020-09-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c0543c24b2242ccf4295c425dd526680 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_50f41ccb62c7389607b0f6ebe5edcafb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4b2ac7c5d7fdcea1d75a8cc42f34dab7 |
publicationDate | 2022-10-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-2022316000-A1 |
titleOfInvention | Nucleic acid detection method by real-time pcr |
abstract | An object of the invention is to provide an optical detection method and a quantification method of a product obtained by amplifying a target nucleic acid contained in a paper piece of a blood spot in a filter paper obtained by blotting blood on a filter paper and then drying the blood using nucleic acid amplification reaction by real-time PCR and to further provide a kit used for the methods. The invention provides a method and a kit which can optically detect and quantify a target nucleic acid from a dried blood spot in a filter paper by real-time PCR without any complicated pretreatment, by adjusting the size of the punched piece of the dried blood spot in a filter paper or the whole blood amount contained in the punched piece, the amount of the PCR reaction reagent, or performing the PCR reaction in a PCR reaction tube closed with a cap. |
priorityDate | 2019-09-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 33.