| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_af18812449a9f384c3c9f5c128d7f09e |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1093
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6876
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1068
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-25 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate |
2020-06-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_308f7f18abd38137319529cee0f2b418
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_429b71bdbed5f2163472b11eec4a6154
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_339bd46fa3f3384827e85df66a9efff8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a69536cdd43d80d4d50cef56f7462359
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ecef909a70f51e97ecbd688b085c0baf |
| publicationDate |
2022-09-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2022298505-A1 |
| titleOfInvention |
Method for constructing pacbio sequencing library |
| abstract |
Provided in the present invention is a method for constructing a PacBio sequencing library, comprising the following steps: (1) obtaining a target double-stranded DNA; (2) adding a thermostable RNA ligase to respectively connect two ends of the double-stranded DNA to form a closed loop to obtain a dumbbell-shaped DNA library; (3) purifying the dumbbell-shaped DNA library; and (4) binding with sequencing primers and adding a DNA polymerase to obtain a PacBio sequencing library. |
| priorityDate |
2019-06-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |