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filingDate 2021-12-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_038b449aee2bf31a391f1298dcaa6706
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publicationDate 2022-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2022204998-A1
titleOfInvention Guanidine degradation enzyme and methods of use
abstract Presented herein are Synechococcus strains engineered to express the bacterial ethylene-forming enzyme (EFE) that exhibit unstable ethylene production due to toxicity and genomic instability induced by accumulation of the EFE-byproduct guanidine. Co-expression of EFE and Sll1077 significantly enhanced genomic stability and enabled the resulting Synechococcus strain GD-EFE7942 to achieve sustained high-level ethylene production. The engineered strains and methods disclosed herein are useful for guanidine degradation pathways and for ethylene bioproduction in cyanobacteria.
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