| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d40285e27f4ed83b8728b0d477404958 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y104-01002
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y104-01004
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y104-01003 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B35-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G06N20-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B15-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B30-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P13-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y101-01047
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0006
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0016 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-06 |
| filingDate |
2021-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fd34f1e112dd958831cdff1a3dcf7fbf
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_57eec26added0d38601af5d777da3ec6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e4254d8215306573ac47bbcfba0bb0bd
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b86785746036cc02157115f6b14fe8ad
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_46f4aca5b83ca74c561f89480d17b744
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_28dc57ea0afe821e0e978fa7123d5346 |
| publicationDate |
2022-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2022204949-A1 |
| titleOfInvention |
Gene mining method combining functional sequence and structure simulation, nadh-preferring phosphinothricin dehydrogenase mutant and application thereof |
| abstract |
Disclosed are a gene mining method combining functional sequence and structure simulation, an NADH-preferring phosphinothricin dehydrogenase mutant and an application thereof. The gene mining method comprises the following steps: (1) analyzing a characteristic sequence which an NADH-type glutamate dehydrogenase should have; (2) searching a gene library based on the characteristic sequence; (3) performing clustering analysis and protein structure simulation on genes obtained by the searching; (4) selecting genes that feature high gene aggregation and a protein structure similar to that of the known phosphinothricin dehydrogenase as candidate genes. A wild-type phosphinothricin dehydrogenase with an amino acid sequence as set forth in SEQ ID No.2 derived from Lysinibacillus composti is obtained through the gene mining, and then mutated, and an NADH-preferring phosphinothricin dehydrogenase mutant is screened out, which has a mutation site selected from one of the following: (1) A144G-V375F-M91A; (2) A144G-V345A-M91A; (3) A144G. This mutant enzyme can be used for catalytic reaction with an inexpensive coenzyme NAD. |
| priorityDate |
2020-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |