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filingDate 2020-11-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2022-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2022195539-A1
titleOfInvention Methods for Detecting Low Levels of Covid-19 Virus
abstract Provided herein is a method for detecting the presence of a COVID-19 virus in a human sample or an environmental sample having one or more viral and bacterial pathogens. Samples processed to obtain total nucleic acids. The nucleic acids are used as a template in a reverse transcription-amplification reaction to obtain cDNA, which is used in a PCR amplification reaction to obtain fluorescent COVID-19 virus specific amplicons. These amplicons are detected by microarray hybridization near the lowest limit of detection. Also provided is a method for detecting in addition to the COVID-19 virus, the presence of respiratory disease-causing pathogens including viruses, bacteria and fungus in a single assay using the above method.
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