| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5bc5292a07e34d16eb1d033a16d3aec3 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R2001-19 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-205
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-52 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 |
| filingDate |
2020-04-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4035bb85fc966d326d98c3dd22b5df03
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_db46f86ac4d3a6e737d894d75f758fdf
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_336d7cf08cc9335b6026dc3baaf91b15
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f2a7218c9a52eae616389655f1aff3e8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d1e5aa3a8284ca20cc6695f62c81f827
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_be3a6abfcd2251d01b9617d077e2c474 |
| publicationDate |
2022-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2022195474-A1 |
| titleOfInvention |
Metabolic engineering of non-pathogenic escherichia coli strains for the controlled production of low molecular weight heparosan and size-specific heparosan oligosaccharides |
| abstract |
Methods for producing heparosan oligosaccharides and polysaccharides include culturing a recombinant host cell of a non-pathogenic E. coli strain, the host cell having been engineered to comprise the biosynthetic gene cluster kfiA, KfiB, kfiC, kfiD and, optionally, the gene elmA in culture conditions enabling direct expression heparosan oligosaccharides of specific sizes and/or of heparosan low molecular weight precursors by the host cell. The methods further includes obtaining such expression products during the culturing. A majority of the heparosan oligosaccharides range in size from approximately tetrasaccharide to approximately dodecasaccharide and a majority of the heparosan polysaccharides range in mass from approximately 5 KDa to approximately 30 KDa. |
| priorityDate |
2019-04-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |