http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2022186239-A1

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filingDate 2021-12-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f1ea8e904c2af480ae5be1d4761be381
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publicationDate 2022-06-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-2022186239-A1
titleOfInvention PEACH POLYGALACTURONASE-INHIBITING PROTEIN PpPGIP1 GENE, AND CLONING METHOD AND USE THEREOF
abstract The present invention discloses a peach polygalacturonase-inhibiting protein PpPGIP1 gene, and a cloning method and use thereof. The peach polygalacturonase-inhibiting protein PpPGIP1 gene has a nucleotide sequence shown in SEQ ID NO: 1, and a protein encoded by the peach polygalacturonase-inhibiting protein PpPGIP1 gene has an amino acid sequence shown in SEQ ID NO: 2. The cloning method includes the following steps: (1) extracting total RNA from a peach, and subjecting the total RNA to reverse transcription to obtain cDNA, which serves as a template; (2) designing primers based on the PpPGIP1 gene sequence; and (3) Polymerase Chain Reaction (PCR) amplification: conducting PCR amplification to obtain a PpPGIP1 gene amplification product. As there is a protein-protein interaction relationship between PpPGIP1 and PpVIN2, the effective inhibition of the PpPGIP1 expression in peach can significantly reduce the activity of the acid invertase PpVIN2 and thus reduce the decomposition of sucrose.
priorityDate 2020-12-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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