http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2022186239-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_89d8e7355750aa815842a3fa5b382c19 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-415 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8205 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8218 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 |
filingDate | 2021-12-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f1ea8e904c2af480ae5be1d4761be381 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4b668ec80e7bc5b4fa3ebdfaced1d741 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f524c6966ba415eb8c943475b3c2b6ad http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e4e38493819bbaec55b8d2b42225aa02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f115960956a5cf389686c79ebeeb1305 |
publicationDate | 2022-06-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-2022186239-A1 |
titleOfInvention | PEACH POLYGALACTURONASE-INHIBITING PROTEIN PpPGIP1 GENE, AND CLONING METHOD AND USE THEREOF |
abstract | The present invention discloses a peach polygalacturonase-inhibiting protein PpPGIP1 gene, and a cloning method and use thereof. The peach polygalacturonase-inhibiting protein PpPGIP1 gene has a nucleotide sequence shown in SEQ ID NO: 1, and a protein encoded by the peach polygalacturonase-inhibiting protein PpPGIP1 gene has an amino acid sequence shown in SEQ ID NO: 2. The cloning method includes the following steps: (1) extracting total RNA from a peach, and subjecting the total RNA to reverse transcription to obtain cDNA, which serves as a template; (2) designing primers based on the PpPGIP1 gene sequence; and (3) Polymerase Chain Reaction (PCR) amplification: conducting PCR amplification to obtain a PpPGIP1 gene amplification product. As there is a protein-protein interaction relationship between PpPGIP1 and PpVIN2, the effective inhibition of the PpPGIP1 expression in peach can significantly reduce the activity of the acid invertase PpVIN2 and thus reduce the decomposition of sucrose. |
priorityDate | 2020-12-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 52.