| abstract |
A method of modifying a gene encoding or processed into a non-coding RNA molecule having no RNA silencing activity in a eukaryotic cell, wherein the gene encoding or processed into the non-coding RNA molecule is positioned in a coding gene, is disclosed. The method comprising introducing into the eukaryotic cell a DNA editing agent conferring a silencing specificity of the non-coding RNA molecule towards a target RNA of interest. Target RNA of interest include, for example, a transcript of a gene selected from the group consisting of a housekeeping gene, a dominant gene, a gene comprising a high copy number, and a gene associated with cell apoptosis. Methods comprising DNA or RNA editing agents which elicit base editing are also disclosed. |