| abstract |
The present disclosure provides compositions and methods for performance of targeted mutagenesis in higher eukaryotic cells, e.g., mammalian cells, across large stretches of targeted sequence. Compositions and methods that rely upon combination of a bacteriophage polymerase with a nucleic acid-editing deaminase to achieve robust mutagenesis of targeted regions of nucleic acid sequence under control of a phage promoter are specifically provided. |