http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2022118113-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fb82523b4b53210f316c795319889b81 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-24068 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-24069 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-50 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-582 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-24069 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K49-0056 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K49-0032 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-24068 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K49-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-52 |
| filingDate | 2020-01-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1071c03314ee91eb6193b43716dd58d6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4e485b0581a622802f1203bdb68a369a |
| publicationDate | 2022-04-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2022118113-A1 |
| titleOfInvention | Sortase-labelled clostridium neurotoxins |
| abstract | The present invention relates to a method for preparing a labelled polypeptide, the method comprising: a. providing a polypeptide comprising: i. a sortase acceptor site or a sortase donor site; ii. a non-cytotoxic protease or a proteolytically inactive mutant thereof; iii. a Targeting Moiety (TM) that is capable of binding to a Binding Site on a target cell; and iv. a translocation domain; b. incubating the polypeptide with: a sortase; and a labelled substrate comprising a sortase donor site or a sortase acceptor site, respectively, and a conjugated detectable label; wherein the sortase catalyses: conjugation between an amino acid of the sortase acceptor site of the polypeptide and an amino acid of the sortase donor site of the labelled substrate; or conjugation between an amino acid of the sortase acceptor site of the labelled substrate and an amino acid of the sortase donor site of the polypeptide; thereby labelling the polypeptide; and c. obtaining the labelled polypeptide. The invention also relates to polypeptides for labelling, labelled polypeptides, nucleic acids encoding said polypeptides, and methods of using and manufacturing said polypeptides. |
| priorityDate | 2019-01-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 818.