| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cb351a5a4e03ee3773bd0df5dbae9ac3 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01021
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R2001-84 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01021
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-815
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2445 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-42
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-14 |
| filingDate |
2021-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3d5017f64671075fe100d234a214254a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_389ada4a69cd74e5fee0b70919486133
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_102c6bcb23067465e7833c03ddc98009
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c09b16afdee55e208901732baa69da18 |
| publicationDate |
2022-02-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2022056496-A1 |
| titleOfInvention |
Preparation of Thermophilic beta-Glucosidase and Application Thereof |
| abstract |
The disclosure discloses the preparation of thermophilic β-glucosidase and the application thereof, which belongs to the technical field of genetic engineering and fermentation engineering. The present disclosure heterologously expresses the β-glucosidase TpBgl3A derived from thermophilic fungus Talaromyces piceae by constructing a recombinant bacteria. The enzyme production of recombinant bacteria can reach 2324 U/mL in a 3.6 L fermenter. The resulting β-glucosidase TpBgl3A can produce gentioligosaccharides with a high conversion rate using glucose as a substrate at a lower enzyme amount added, which significantly reduces production costs. In the reaction system using glucose and cellobiose as substrates, the conversion rate of gentioligosaccharide reaches 26.2%, which has the potential for industrial production of gentioligosaccharide. |
| priorityDate |
2020-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |