http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2022049280-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cb351a5a4e03ee3773bd0df5dbae9ac3 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1029 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y203-01004 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-74 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-10 |
| filingDate | 2019-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_de2ca0225c9445cb07131cc2a76bd00a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_30809e89113599228a39b065b0ae1c12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5a782018a51edbf5c2505fc6b1b962ad http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fd6612316303a1c8b6adebfe93271f9e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_85b383a68e3b72e8b4fe49671460dab7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2070b1ce27a242c020a75e0b8779d447 |
| publicationDate | 2022-02-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2022049280-A1 |
| titleOfInvention | Method for promoting n-acetylglucosamine synthesis by using glcn6p responsive element |
| abstract | The present invention provides a method for promoting N-acetylglucosamine synthesis by using the GlcN6P responsive element. In the present invention, Bacillus subtilis BSGNY-Pveg-glmS-P43-GNA1 is used as a starting strain, in which a CRISPRi system regulated by GlcN6P responsive element is integrated into the genome to dynamically weaken the N-acetylglucosamine synthesis competitive pathway; a GlcN6P responsive promoter is used to regulate the expression of GNA1 on the plasmid to dynamically regulate the N-acetylglucosamine synthesis pathway; and the key gene alsSD involved in the acetoin synthesis pathway is knocked out. During fed-batch fermentation with this strain in a 15 L fermenter, the production of N-acetylglucosamine reaches 131.6 g/L and no by-product acetoin is accumulated, which lays a foundation for the production of GlcNAc by industrial fermentation. |
| priorityDate | 2019-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 104.