| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f9a490d8192284884d8e192f29cb7dd6 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-186
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-113
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-3231
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2527-107
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2537-163 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6876
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-11 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6876
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6853 |
| filingDate |
2019-12-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f305c37eacdd584475ad8207faa25214
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0a7d1327fe3c4d064b1a666818de2c34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e8ca1c06a1b20605bffa479ac462f163
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1dfe0e9889108b960d338fa271f1eafc |
| publicationDate |
2022-01-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-2022025452-A1 |
| titleOfInvention |
Nucleic acid amplification blocking agent for detecting low-abundance mutation sequence and use thereof |
| abstract |
A nucleic acid amplification blocker for detecting a low-abundance mutation sequence and an application thereof in detecting a low-abundance mutation sequence are provided. The nucleic acid amplification blocker is an oligonucleotide modified by locked nucleic acid (LNA), and the matching region of the nucleic acid amplification blocker is located between amplified sequences. The nucleic acid amplification blocker is completely complementary to wild-type gene sequence, and contains at least one mismatch with mutant sequence. The nucleic acid amplification blocker has a great difference in affinity with mutant nucleic acid sequence/wild-type nucleic acid sequence, so as to achieve the purpose of highly selective amplification/enrichment of mutant sequence in samples. The nucleic acid amplification blocker has more significant detection effect on deletion mutation and insertion mutation. |
| priorityDate |
2018-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |