http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2022002797-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cf8a3231cd88b81a086ffdc933fdef2c |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 |
filingDate | 2019-08-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ac85b18eec4f57e07b14160b84d0a637 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_96920d09465723d2301e06f549a3d9e3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5b55244cce3d80d2fa9e76477c06dfc9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fe21e68aac005c1c145cea6d0c4656fe http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_541b5d5bee3cccf62c73e3c06b0441e6 |
publicationDate | 2022-01-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-2022002797-A1 |
titleOfInvention | Full-length rna sequencing |
abstract | The present disclosure provides a method for analyzing a polyribonucleotide, wherein the polyribonucleotide is an mRNA molecule with a poly(A) tail, said method comprising: (a) obtaining a labeled polyribonucleotide by GI tailing; followed by (b) providing a second molecule comprising a first primer recognition sequence followed by a sequence of C nucleotide residues linked to a sequence of T nucleotide residues; followed by (c) obtaining a complex of said labeled polyribonucleotide and said second molecule; followed by (d) obtaining an extended second molecule by (d1) extending the 3′ end of the second molecule by synthesizing a sequence that is complementary to the labeled polyribonucleotide; followed by (d2) extending the 3′ end of the second molecule by adding at least 1 to 5 C nucleotide residues, followed by a second primer recognition sequence; said method providing information on entire polyribonucleotides, in particular mRNA isoforms and their respective poly(A) tails. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114582419-A |
priorityDate | 2018-10-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 72.