Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_53c911e75ea09dd4c2705db5306dae77 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6876 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6848 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6876 |
filingDate |
2019-10-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_40082908623359aa22cfa0045076c6a0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0a232ac4a6aac1481ff509362154e363 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_990b7b262053b8526e198ef611842c2a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fc7398e47e2007081a4367d407a956ef http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fa8f687f73219288c60b637409799831 |
publicationDate |
2021-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-2021388427-A1 |
titleOfInvention |
Liquid sample workflow for nanopore sequencing |
abstract |
The present invention relates to a method of characterizing a target DNA polynucleotide using rolling circle amplification (RCA) and a synthetic single guide RNA (sgRNA) to identify and cleave the WT version of the target DNA polynucleotide. Also provided are characterization steps based on the use of a transmembrane pore and a DNA translocase enzyme controlling the movement of the DNA polynucleotide through the transmembrane pore. Further envisaged is a kit comprising one or more oligonucleotides specific for at least a portion of the target DNA polynucleotide, an sgRNA specific for the WT version of the target DNA polynucleotide and an sgRNA-guided nucleic acid-binding protein. |
priorityDate |
2018-11-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |