| abstract |
Disclosed is an enhanced method for rapid and cost-effective analysis of sequences of a microorganism by qPCR. These methods identify allelic variation, SNPs, and genetic mutations of a particular gene such as those responsible for conferring resistance or sensitivity to an antibiotic, chemotherapy, or another chemical compound. By selection of appropriate gene regions, mutation loci that confer resistance to key antibiotics can be identified by qPCR. Additionally, the approach can identify heteroresistant strains, e.g., populations of strains from a sample that contain both mutation and wild-type nucleotides. By selecting appropriate that bind efficiently to the area of mutation can identify resistance conferring mutations. Methods are useful to sequences derived from viral agents, such as influenza virus, bacterial agents, such as tuberculosis bacteria, and cancer cells. |