| abstract |
The present invention relates to the field of analysis of traditional Chinese medicine, and in particular to a method of establishing a fingerprint spectrum of a traditional Chinese medicine composition with effect of regulating depression emotion or a formulation thereof, wherein the traditional Chinese medicine composition consists of the following traditional Chinese medicine extracts in parts by weight: 40˜80 parts of Hypericum perforatum extract, 10˜40 parts of Acanthopanax senticosus extract, and 2˜20 parts of tree peony root bark extract; and the method comprises establishing the fingerprint spectrum by high performance liquid chromatography, and the chromatographic conditions are as follows: an octadecylsilane bonded silica gel chromatographic column is used as a chromatographic column; column temperature is 25˜35° C.; flow rate is 0.5˜1.1 mL/min; detection wavelength is 290˜310 nm; mobile phase A is phosphoric acid aqueous solution, mobile phase B is acetonitrile, and a gradient elution program is used. |