http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2021261952-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_45feb2e2d7e39d6d57d5e303cfe69941 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-117 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-101 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-173 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2535-122 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-204 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-107 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6876 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1093 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6876 |
filingDate | 2021-02-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_53bf5459eead508f07711e6c3d0c04a3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2c0f6e6e22beb4827bc6629d76c38566 |
publicationDate | 2021-08-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-2021261952-A1 |
titleOfInvention | Compositions and methods for generating massively parallel nucleic acid sequencing libraries |
abstract | Oligonucleotide primers and methods of use in producing sequencing libraries are provided according to aspects of the present disclosure which include, from 5′ to 3′, a homopolymer-hybridizing region, and an anchor region comprising 5′-(λ) n N m -3′, wherein the homopolymer-hybridizing region is a contiguous sequence of 5 to 20 elements, wherein the elements are nucleotides or T m increasing nucleotide analogs, wherein at least 4 of the elements are T m increasing nucleotide analogs, wherein the homopolymer-hybridizing region hybridizes to a complementary homopolymer tract of a target nucleic acid, wherein the complementary homopolymer tract comprises a contiguous sequence of complementary elements, wherein λ is any nucleotide or nucleotide analog with the proviso that λ is not a nucleotide or nucleotide analog complementary to a complementary element of the complementary homopolymer tract, and wherein N is any nucleotide or nucleotide analog. |
priorityDate | 2020-02-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 192.