| abstract |
Provided herein are methods for analyzing a target nucleic acid, comprising contacting a target nucleic acid with a first polynucleotide and a second polynucleotide, and optionally one or more other polynucelotides such as a splint and/or a primer, to form a hybridization complex. In some embodiments, the first polynucleotide and the second polynucleotide are ligated to form a circular polynucleotide hybridized to the target nucleic acid, e.g., using DNA-templated ligation reaction(s). The circular polynucleotide and/or a product thereof (e.g., an RCA product) can be analyzed to analyze the target nucleic acid or a sequence thereof. |