abstract |
Disclosed herein include systems, methods, compositions, and kits for 5′-based gene expression profiling and for whole transcriptome analysis (WTA) with random priming and extension (RPE). Blocker oligonucleotides capable of specifically binding to a portion of an oligonucleotide barcode are provided in some embodiments. The blocker oligonucleotides can reduce of the generation of undesirable extension products, such as, for example, the extension products of random primers hybridized to a portion of the oligonucleotide barcode. Immune repertoire profiling methods are also provided in some embodiments. |