| abstract |
The disclosure discloses a tissue-engineered nerve transplant and a preparation method thereof, and belongs to the technical fields of biomaterials and tissue engineering. By optimizing the specification of stripes, the stripes can independently induce EMSCs to differentiate to myelination cells (Schwann cells) to the maximum extent so as to obtain an EMSCs/biomaterial scaffold compound. The EMSCs/biomaterial scaffold compound can not only be used as a three-dimensional cell culture model for researching neural stem cell differentiation, nerve fiber growth and myelination molecular mechanisms in vitro, but also be used as a tissue engineering transplant for in-vivo transplantation to repair nervous system injury. In the disclosure, an EMSCs/micropatterned biomaterial film is rolled into a cylindrical multi-tunnel type nerve regeneration conduit to be used to repair sciatic nerve injury by transplantation, and results show that the disclosure can promote nerve regeneration and recovery of a lower limb motor function through injured portion transplantation, and has good clinical application prospects and research and development value. |