http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2021144977-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_161663de5408e572e0d7c6b04dfabc7a |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0068 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-033 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-10 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-033 |
| filingDate | 2018-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f12183b26a9dcc6f0264f369019de87e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b6bfa7507d9cbec1e1a489c4057e4fe5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_27bf516acf327c1c2ceee61bbb598265 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f335dbae3ad66e630a0c9369a3658dec http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_322f480de4134e7fe4bb9efecf7a073e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_63f0e64b1e43b4cdda54cb76491e143f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b58d9a754c9328fa9c14435c579192ff |
| publicationDate | 2021-05-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-2021144977-A1 |
| titleOfInvention | Medium for culturing Balantidium ctenopharyngodoni in vitro, method for preparing the medium and method for culturing Balantidium ctenopharyngodoni in vitro |
| abstract | A medium for culturing Balantidium ctenopharyngodoni in vitro, method for preparing the medium and method for culture in vitro are provided, which belongs to technical field of in vitro culture of intestinal protozoa. The formulation of the culture medium includes: 100 ml of Ringer's solution, 0.5 g of yeast extract, 1.0 g of proteose peptone, 3-6 ml of fetal bovine serum, 6-10 ml of horse serum, 300-500 μl of Bacillus licheniformis suspension, 200-300 mg of aseptic starch. Culture steps include: inoculating collected Balantidium ctenopharyngodoni into a prepared medium, filling with nitrogen and sealing, culturing at 15° C. for 48-72 hours, then transferring to another fresh medium, cycling back and forth, proliferating the Balantidium ctenopharyngodoni continuously. Balantidium ctenopharyngodoni are capable of achieving cell division and proliferation in the culture medium, and lays foundation for the physiological and experimental ecology research of the Balantidium ctenopharyngodoni. |
| priorityDate | 2018-04-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 48.